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A healthy Amazon benefits everyone on the planet, especially those who live and work in the region. But the region faces serious threats: over the last four decades carbon uptake, biodiversity, and ecosystem productivity have declined. Climate change; expansion of human settlements; unsustainable energy and agricultural development; mineral extraction; and criminal activities such as farming illicit crops, illegal logging, gold mining, and land trafficking increasingly threaten the region’s forests. The Amazon’s remarkable biodiversity—accounting for about one-third of all known plant, animal, and insect species—is also under threat.1 Alarmingly, parts of the Amazon rainforest have transitioned from carbon sinks to carbon sources and are now emitting more carbon than they sequester.2 Scientists fear the deforestation of the Amazon will lead to catastrophic effects elsewhere on the planet that would be impossible to reverse.3 To address these threats and preserve the region’s ecosystems, the United States Agency for International Development (USAID) developed the Amazon Vision in 2016. This framework establishes a concerted and strategic regional response across USAID’s efforts in Brazil, Colombia, Peru, Ecuador, Guyana, and Suriname. The Vision describes, guides, and measures USAID’s investments and conservation impact at a regional scale and encompasses the work of the Amazon Regional Environment Program (AREP), bilateral Missions, and USAID/ Washington projects in the Amazon. The purpose of this report is to share progress toward the Amazon Vision’s ongoing efforts to maintain a healthy and resilient Amazon basin. This document describes the challenges and accomplishments of USAID’s biodiversity and sustainable landscape initiatives through this lens.